Development of an innovative technology for sorting sperm in order to obtain cattle of a specific sex

Technology of sorting bull sperm tto obtain female calves by determining the effect of gold nanoparticles on sperm structures.
Achieving the goals involves obtaining dairy heifers with a significant reduction in semen costs and a higher female fertilization rate.

1. Minimizing sperm space;
2. Higher fertilization potential of cells, increase in calving efficiency, loss of embryos;
3. Enhanced X sperm equipment in load;
4. Maximizing the number of female calves born;
5. Possibility of using nano gold for sperm sorting;
6. Lower cost of producing insemination doses, eliminating the costs of producing a female.

Project activities

Cytometric, biochemical and computer-assisted analysis of bull's sperm. The influence of gold nanoparticles on cell viability, DNA integrity, acrosome integrity, mitochondrial potential, oxidative stress parameters and sperm motility.

The main benefits of the project

Effects:

1. Innovative technology with high implementation potential (application value)
2. Increased competitiveness with respect to other commercial sperm sorting technologies (economic value)
3. Reducing the costs of obtaining replacement females (economic value)
4. Understanding the mechanisms of influence of gold nanoparticles on sperm cells, the effectiveness of fertilisation, and the development of early pregnancy (scientific value)

Practice summary

The necessity to develop a new technology results from the need to obtain a highly effective sorted product, available to a larger group of farmers at an affordable price, which is of great importance, especially considering today's milk production costs. Implementing this technology will allow for high semen effectiveness and will significantly increase the rate of conception, reducing the loss of embryos by limiting the negative effects of sperm separation. This global breakthrough technology will allow for increased genetic progress in breeding herds and insemination stations, which will undoubtedly have a positive impact on the European, and in particular the Polish, insemination and breeding market.

Results achieved

Technology of sorting bull sperm to obtain female calves by determining the effect of gold nanoparticles on sperm structures.

Achieving the goals involves obtaining dairy heifers with a significant reduction in semen costs and a higher female fertilization rate.

Effects:

1. Innovative technology with high implementation potential (application value);
2. Increased competitiveness with recpect to other commercial sperm sorting technologies (economic value);
3. Reducing the cost of obtaining replacement females (economic value);
4. Understanding the mechanisms of influence of gold nanparticles on sperm cells, the effectiveness of fertilisation, and the development of early pregnancy (scientific value).

The research conducted opens up new perspectives for the development of sperm sorting technology with potential benefits for the breeding industry.

• Result 1 - minimizing sperm space;
• Benefit 1 - higher fertilization potential of cells, increase in calving efficiency, loss of embroys;

• Result 2 - enhanced X sperm equipment in load;
• Benefit 2 - Maximizing the number of female calves born;

• Result 3 - Possibility of using nano gold for sperm sorting;
• Benefit 3 - lower cost of producing insemination doses, eliminating the costs of producing a female.